Composite

Part:BBa_K1618021:Design

Designed by: Kieran Rustage   Group: iGEM15_NRP-UEA-Norwich   (2015-08-28)


Plac-THL-CRO-BHBD-BCD-EFTb-EFTa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 3159
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 3159
    Illegal NheI site found at 1167
    Illegal NotI site found at 235
    Illegal NotI site found at 3841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal EcoRI site found at 3159
    Illegal BglII site found at 1415
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 3159
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 229
    Illegal EcoRI site found at 3159
    Illegal XbaI site found at 244
    Illegal AgeI site found at 1338
    Illegal AgeI site found at 3354
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

.


Source

Coprococcus sp. L2-50

References